Release of muscle alpha-actin into serum after intensive exercise

Purpose: To study the effects of high-level matches on serum alpha actin and other muscle damage markers in teams of rugby and handball players. Methods: Blood samples were drawn from 23 sportsmen: 13 rugby players and 10 handball players. One sample was drawn with the player at rest before the match and one immediately after the match. Immunoassays were used to determine troponin I, troponin T, LDH, and myoglobin concentrations. Western blot and densitometry were used to measure alpha-actin concentrations. Muscle injury was defined by a total CK value of > 500 IU/L (Rosalki method). Results: Mean pre- and post-match serum alpha-actin values were, respectively, 7.16 and 26.47 mikro-g/ml in the handball group and 1.24 and 20.04 mikro-g/ml in the rugby team. CPK, LDH and myoglobin but not troponin 1 levels also significantly differed between these time points. According to these results, large amounts of alpha-actin are released into peripheral blood immediately after intense physical effort. Possible cross-interference between skeletal and cardiac muscle damage can be discriminated by the combined use of alpha-actin and troponin I. Conclusion: The significant increase in alpha-actin after a high-level match may be a reliable marker for the early diagnosis and hence more effective treatment of muscle injury.
© Copyright 2010 Biology of Sport. Termedia Publishing House. Published by Institute of Sport Warsaw. All rights reserved.

Subjects: handball rugby training effect analysis immunity blood muscle heart injury
Notations: biological and medical sciences sport games
Published in: Biology of Sport
Published: Warsaw Institute of Sport Warsaw 2010
Volume: 27
Issue: 4
Pages: 263-268
Document types: article
Language: English
Level: advanced