The muscles of the deep stabilization system (DSS) affect spinal stabilization, and correct muscular coordination as a prerequisite for a centered position of the joints with optimal biomechanics of movement. Its stimulation by special exercises might lead to better performance in sports. The study verifies the effect of an 8-week intervention to activate DSS on the shot speed in a group of 15 adult elite female handball players. 30 participants were distributed to either the Control group (CG) or the Experimental group (EG). The EG involved special blocks with exercises activating DSS included in regular handball training. The CG involved only casual training. Input and output measurements included shot velocity assessment from various positions with a Stalker Sports Radar Gun. Mann-Whitney U-test was utilized for the statistical analysis with a 0.05 level of significance. The experiment showed a significant speed increase for all three tested throws. Speed increased by 3.82% for a one-handed overhead shot from the ground from a distance of 7m (free throw), 2.23% for a one-handed overhead shot from the ground after a run-up from a distance of 9m, and 2.23% for a one-handed overhead shot from a jump from a distance of 9 m by 2.38%. Specific activation exercises of DSS of the spine led to increased shot speed in handball.
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