The effect of maximal physical exercise on relationships between the growth hormone (GH) and insulin-like growth factor 1 (IGF-1) and transcriptional activity of CYP1A2 in young ice hockey players
(Die Auswirkung maximaler körperlicher Belastung auf das Verhältnis zwischen Wachstumshormonen und dem IGF-1 und der transkriptionallen Aktivität des CYP1A2 bei jungen Eishockeyspielern)
BACKGROUND: The maximal physical exercise influences on changes in GH, IGF-1 concentrations and activity of CYP1A2. The aim of this study was to answer the question whether the change of endogenous GH and IGF-1 influenced the expression of CYP1A2 and whether these changes correlate with others parameters of blood and test.
METHODS:
The twenty ice young hockey players were subjected to maximal exercise cycle test. The test duration was mean 21.0±2.4 min, and work done was mean 3261.3±558.3 J/kg. In blood samples collected before and after the exercise were evaluated: concentrations of GH and IGF-1 which were determined in the serum by IRMA method and the transcriptional activity of CYP1A2 in RNA isolated from whole blood by the QRT-PCR reaction.
RESULTS:
Before the exercise the median of GH was mean 0.31±0.23 µU/mL and after the exercise increased to mean 21.6±16.6 µU/mL (P<0.001). Mean of serum IGF-1 level before the exercise and immediately after test did not change. Statistically significant increase in value of Ct for CYP1A2 (P<0.001) was found.
CONCLUSION:
The results of the study showed that: an intense exercise of young ice hockey players led to a significant increase in GH concentration, which caused the Ct values of CYP1A2 increase, whereas IGF-1 did not change. GH and IGF-1 levels after the exercise depended on the work done and the relative levels of CYP1A2 expression correlated with the time and the amount of work done by the athletes.
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Schlagworte: | Relation Belastung Hormon Nachwuchsleistungssport Eishockey |
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Notationen: | Spielsportarten Biowissenschaften und Sportmedizin |
Tagging: | Wachstumshormon IGF-1 |
Veröffentlicht in: | The Journal of Sports Medicine and Physical Fitness |
Veröffentlicht: |
2015
|
Jahrgang: | 55 |
Heft: | 3 |
Seiten: | 158-163 |
Dokumentenarten: | Artikel |
Sprache: | Englisch |
Level: | hoch |